Signalling

Part:BBa_K2323003:Design

Designed by: Jorge Andres Quintero Serna   Group: iGEM17_Munich   (2017-10-25)


GFP-pdt2E


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


Design Notes

This protein degradation tag is part of a collection, which is easy to clone to tag any protein using overhang PCR.


Source

This degradation tag is taken from Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014. It can be degraded at a slow rate (0.0046/min) by the ClpP machinery of E.coli, but also at a faster rate by an exogenous protease (mf-lon).

References

Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014